5BZB
NavMs voltage-gated sodium channel pore and C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92001 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.340, 332.763, 80.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.810 - 2.700 |
| R-factor | 0.1801 |
| Rwork | 0.178 |
| R-free | 0.21420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zjz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.29) |
| Phasing software | BUSTER (2.10.2) |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.810 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.245 | 0.844 |
| Number of reflections | 30149 | |
| <I/σ(I)> | 9.5 | 3.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.6 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1m na3citrate, 0.1m tris ph 8.0, 34% peg 400 |
