5BZ9

X-ray crystal structure of a continuously hydrogen bonded 14mer DNA lattice.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77.15
Detector technology	PIXEL
Collection date	2014-06-29
Detector	PSI PILATUS 6M
Wavelength(s)	0.979200
Spacegroup name	P 31 2 1
Unit cell lengths	25.989, 25.989, 121.531
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.510 - 2.100
R-factor	0.27493
Rwork	0.271
R-free	0.31152
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bxw
RMSD bond length	0.006
RMSD bond angle	1.406
Data scaling software	XDS
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.510	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.138	0.244
Number of reflections	3035
<I/σ(I)>	16.3	3
Completeness [%]	97.8	97.4
Redundancy	10.6	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296.15	120mM Magnesium Formate 50mM Lithium Chloride 10% MPD