5BY3
A novel family GH115 4-O-Methyl-alpha-glucuronidase, BtGH115A, with specificity for decorated arabinogalactans
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795, 0.9798, 0.9682 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.770, 102.300, 122.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.440 |
| R-factor | 0.19326 |
| Rwork | 0.189 |
| R-free | 0.26583 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.848 |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.510 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.614 | |
| Number of reflections | 32214 | |
| <I/σ(I)> | 17.6 | 2.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 291 | 20% PEG 3350 0.2M sodium sulfate 0.01M glycine |
