5BXW
X-ray crystal structure of a continuously hydrogen bonded 14mer DNA lattice.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77.15 |
Detector technology | PIXEL |
Collection date | 2014-06-29 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979200 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 25.827, 25.827, 123.080 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.030 - 2.150 |
R-factor | 0.24624 |
Rwork | 0.244 |
R-free | 0.26275 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.887 |
Data scaling software | Aimless |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.030 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.149 | 0.282 |
Number of reflections | 2965 | |
<I/σ(I)> | 14.7 | 2.5 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.8 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296.15 | 120mM Magnesium Formate 50mM Lithium Chloride 10% MPD |