5BXH

Crystal structure of pentameric KCTD9 BTB domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	105.396, 105.396, 97.316
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.566 - 2.760
R-factor	0.2367
Rwork	0.234
R-free	0.27990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3drz
RMSD bond length	0.003
RMSD bond angle	0.736
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.570	2.858
High resolution limit [Å]	2.760	2.760
Rmerge	0.057	0.829
Number of reflections	16374
<I/σ(I)>	10.09	2.46
Completeness [%]	99.7	99.63
Redundancy	9.8	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	25 mg/mL KCTD9 BTB domain with: 0.17 M Lithium citrate, 16% w/v PEG 3350. 1:1 ratio