5BXD
Crystal structure of pentameric KCTD1 BTB domain form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.064, 90.839, 127.694 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.080 - 1.796 |
| R-factor | 0.2036 |
| Rwork | 0.203 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3drz |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.829 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.080 | 1.860 |
| High resolution limit [Å] | 1.796 | 1.796 |
| Rmerge | 0.022 | 0.300 |
| Number of reflections | 50809 | |
| <I/σ(I)> | 16.98 | 2.37 |
| Completeness [%] | 99.8 | 99.52 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25 mg/mL KCTD1 BTB domain with: 0.1 M MES pH 5, 10% w/v PEG 6000. 1:1 ratio |
