5BWL
Crystal Structure of SIRT5 in Complex with a Coumarin-Labelled Succinyl Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 42.420, 55.536, 124.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.071 - 1.548 |
R-factor | 0.2133 |
Rwork | 0.212 |
R-free | 0.23590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nyr |
RMSD bond length | 0.018 |
RMSD bond angle | 1.403 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.580 |
High resolution limit [Å] | 1.548 | 1.550 |
Rmerge | 0.060 | |
Number of reflections | 43833 | |
<I/σ(I)> | 25.3 | 6.9 |
Completeness [%] | 97.7 | 100 |
Redundancy | 4.59 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Bis-Tris (pH 6.5), 20% PEG 3350 |