5BUN
Crystal structure of an antigenic outer membrane protein ST50 from Salmonella Typhi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-25 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 4 |
| Unit cell lengths | 246.847, 246.847, 65.676 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.935 - 2.980 |
| R-factor | 0.213 |
| Rwork | 0.210 |
| R-free | 0.27510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ek9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.931 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.980 |
| Rmerge | 0.110 |
| Number of reflections | 40826 |
| <I/σ(I)> | 10.5 |
| Completeness [%] | 99.8 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 10% PEG 8000, 100mM NaCl, 0.5% |
