5BU4

RIBONUCLEASE T1 COMPLEX WITH 2'GMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-02-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	40.470, 48.100, 50.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.770
R-factor	0.172 *
Rwork	0.172
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7gsp
RMSD bond length	0.013 *
RMSD bond angle	2.354
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	RESTRAIN

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.770
Rmerge	0.188 *	0.978 *
Total number of observations	380597 *
Number of reflections	9833
<I/σ(I)>	23.64
Completeness [%]	93.8
Redundancy	38.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.2		Zegers, I., (1998) Nat. Struct. Biol., 5, 280. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	2'GMP	2 (mM)
3	1	drop		20 (mM)
4	1	drop		2 (mM)
5	1	reservoir	MPD	55 (%(v/v))