5BU2
Structure of the C-terminal domain of lpg1496 from Legionella pneumophila in complex with nucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9770 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.867, 69.707, 77.027 |
| Unit cell angles | 89.87, 72.30, 70.31 |
Refinement procedure
| Resolution | 34.000 - 2.110 |
| R-factor | 0.23123 |
| Rwork | 0.229 |
| R-free | 0.27307 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bu1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.212 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.435 | |
| Number of reflections | 54209 | |
| <I/σ(I)> | 11.3 | 1.7 |
| Completeness [%] | 95.0 | 78.3 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES, 25% (w/v) PEG3350, 5 mM ADP |
