5BTZ
Structure of the middle domain of lpg1496 from Legionella pneumophila in P212121 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-31 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9770 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.137, 44.066, 77.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.780 - 1.600 |
| R-factor | 0.20124 |
| Rwork | 0.200 |
| R-free | 0.23125 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.031 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.406 | |
| Number of reflections | 16100 | |
| <I/σ(I)> | 49.7 | 4.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.6 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES, 25% (w/v) PEG3350 |
