5BTQ
Crystal structure of human heme oxygenase 1 H25R with biliverdin bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.700, 54.740, 72.510 |
| Unit cell angles | 90.00, 99.37, 90.00 |
Refinement procedure
| Resolution | 43.500 - 2.080 |
| R-factor | 0.18503 |
| Rwork | 0.183 |
| R-free | 0.22818 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n45 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.906 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.500 | 2.190 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.079 | 0.407 |
| Number of reflections | 26949 | |
| <I/σ(I)> | 14.4 | 3.5 |
| Completeness [%] | 93.3 | 75.3 |
| Redundancy | 5.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 100mM HEPES 2.1M Ammonium sulfate 0.9% 1,6-hexanediol |
