5BTL

Crystal structure of a topoisomerase II complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	107.938, 83.047, 128.737
Unit cell angles	90.00, 108.88, 90.00

Refinement procedure

Resolution	49.600 - 2.500
R-factor	0.231
Rwork	0.230
R-free	0.25550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bs8
RMSD bond length	0.004
RMSD bond angle	0.687
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.600		2.589
High resolution limit [Å]	2.500	11.160	2.500
Rmerge	0.134	0.023
Rmeas	0.156	0.032	1.778
Total number of observations	250907
Number of reflections	250735	1538	9278
<I/σ(I)>	8.38	24.63	0.91
Completeness [%]	98.7	92.2	97.11
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	100 mM Tris-HCl, 13-15% PEG 4000, 200 mM MgCl2, 6.25% PEG 400