5BTE
Crystal structure of Ashbya gossypii Rai1 in complex with pU(S)6-Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.655, 79.742, 160.143 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.410 - 2.400 |
| R-factor | 0.19864 |
| Rwork | 0.196 |
| R-free | 0.25379 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.583 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.124 | 0.465 |
| Number of reflections | 33896 | |
| <I/σ(I)> | 15.3 | 3.6 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 6.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | AgRai1-pU(S)6-Mn2+ complex was obtained by soaking the free AgRai1 crystal with 10mM pU(s)6 and 10mM MnCl2 overnight in the presence of 20%(w/v) PEG3350 and 10%(v/v) ethylene glycol |
