5BTC
Crystal structure of a topoisomerase II complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.336, 82.976, 129.703 |
| Unit cell angles | 90.00, 109.06, 90.00 |
Refinement procedure
| Resolution | 47.699 - 2.550 |
| R-factor | 0.2256 |
| Rwork | 0.224 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bs8 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.696 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.700 | 2.641 | |
| High resolution limit [Å] | 2.550 | 11.400 | 2.550 |
| Rmerge | 0.110 | 0.027 | |
| Rmeas | 0.136 | 0.038 | 1.404 |
| Total number of observations | 239756 | ||
| Number of reflections | 239725 | 1427 | 9517 |
| <I/σ(I)> | 8.98 | 21.3 | 1.26 |
| Completeness [%] | 98.6 | 91.4 | 98.73 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 100 mM Tris-HCl, 13-15% PEG 4000, 200 mM MgCl2, 6.25% PEG 400 |
