5BSZ
X-ray structure of the sugar N-methyltransferase KedS8 from Streptoalloteichus sp ATCC 53650
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-02 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 63.254, 81.004, 126.916 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.710 - 2.000 |
| R-factor | 0.17062 |
| Rwork | 0.168 |
| R-free | 0.22577 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bxo |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.950 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.045 | 0.143 |
| Number of reflections | 21803 | |
| <I/σ(I)> | 14.3 | 4.2 |
| Completeness [%] | 97.6 | 94 |
| Redundancy | 3.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 28-33% MPD, 2.5 mM SAH, 100 mM MOPS |
