5BS7
Structure of histone H3/H4 in complex with Spt2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 121.205, 121.205, 118.503 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 121.200 - 3.300 |
R-factor | 0.23282 |
Rwork | 0.230 |
R-free | 0.29025 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aoi |
RMSD bond length | 0.014 |
RMSD bond angle | 1.760 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 121.200 | 3.360 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.093 | |
Number of reflections | 13876 | |
<I/σ(I)> | 21.6 | 1.1 |
Completeness [%] | 99.0 | 82.4 |
Redundancy | 10.2 | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 0.02 M NaCl, 0.2 M HEPES 7.5, 1.5 M ammonium sulfate |