5BS1
Crystal structure of RbcX-IIa from Chlamydomonas reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.133, 52.989, 61.561 |
| Unit cell angles | 76.49, 81.10, 70.10 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.1787 |
| Rwork | 0.177 |
| R-free | 0.20600 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.509 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.870 | 48.870 | 1.580 |
| High resolution limit [Å] | 1.498 | 4.730 | 1.498 |
| Rmerge | 0.033 | 0.308 | |
| Rmeas | 0.087 | ||
| Rpim | 0.053 | 0.045 | 0.273 |
| Total number of observations | 137620 | 5137 | 7139 |
| Number of reflections | 56366 | ||
| <I/σ(I)> | 10.7 | 24.2 | 2.4 |
| Completeness [%] | 84.2 | 98.6 | 36.8 |
| Redundancy | 2.4 | 2.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 5 % PEG-3350, 0.2 M MgCl2, 50 mM Tris-HCl pH 8.0 |
