5BRK
pMob1-Lats1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.833, 73.338, 53.910 |
| Unit cell angles | 90.00, 99.06, 90.00 |
Refinement procedure
| Resolution | 41.057 - 2.300 |
| R-factor | 0.1892 |
| Rwork | 0.186 |
| R-free | 0.22050 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.798 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.134 | 0.468 |
| Number of reflections | 15456 | |
| <I/σ(I)> | 16.51 | 0.428 |
| Completeness [%] | 95.9 | 80.1 |
| Redundancy | 8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.2 M di-Ammonium citrate 20% (W/V) PEG 3,350 |
