5BRJ

Structure of the bacteriophytochrome response regulator AtBRR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-18
Detector	RAYONIX MX-225
Wavelength(s)	0.97872
Spacegroup name	P 41 2 2
Unit cell lengths	41.046, 41.046, 187.713
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.896 - 1.922
R-factor	0.2002
Rwork	0.196
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	RtBRR
RMSD bond length	0.007
RMSD bond angle	1.029
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.950
High resolution limit [Å]	1.920	1.920
Rmerge	0.051	0.155
Number of reflections	13163
<I/σ(I)>	53.2	23
Completeness [%]	98.7	97
Redundancy	27.2	26.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	200 mM magnesium chloride, 100 mM tris pH 8.5, 20% PEG 8000