5BRA

Crystal Structure of a putative Periplasmic Solute binding protein (IPR025997) from Ochrobactrum Anthropi ATCC49188 (Oant_2843, TARGET EFI-511085)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-18
Detector	RAYONIX MX225HE
Wavelength(s)	0.97931
Spacegroup name	P 41 21 2
Unit cell lengths	81.204, 81.204, 103.629
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.615 - 2.971
R-factor	0.1843
Rwork	0.179
R-free	0.29510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d02
RMSD bond length	0.010
RMSD bond angle	1.386
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	23.800	23.800	3.020
High resolution limit [Å]	2.970	7.960	2.970
Rmerge	0.107	0.073	0.982
Rmeas	0.109	0.074	0.999
Rpim	0.020	0.014	0.184
Total number of observations	215050
Number of reflections	7605
<I/σ(I)>	11.3
Completeness [%]	100.0	100	100
Redundancy	28.3	24.2	29.1
CC(1/2)		0.997	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	Protein (20 mM HEPES, pH 7.5, 5 mM DTT, 10 mM D-ribulose); Reservoir (2.5M Ammonium sulphate, 0.1M TRIS, pH 8.5); Cryoprotection (80% Lithium sulfate, 20% Reservoir)