5BR8
Ambient-temperature crystal structure of 30S ribosomal subunit from Thermus thermophilus in complex with paromomycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2014-11-17 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 1.29 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 401.300, 401.300, 176.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.840 - 3.400 |
| R-factor | 0.1931 |
| Rwork | 0.193 |
| R-free | 0.23360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dr2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.859 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1938) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.840 | |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.290 | 0.870 |
| Number of reflections | 195895 | |
| <I/σ(I)> | 3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | MPD |
