5BR2
Structure of bacteriorhodopsin crystallized from ND-MSP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.973 |
| Spacegroup name | P 63 |
| Unit cell lengths | 61.030, 61.030, 110.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.600 - 1.800 |
| R-factor | 0.19541 |
| Rwork | 0.193 |
| R-free | 0.23679 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.041 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.600 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.069 | 1.045 |
| Number of reflections | 21165 | |
| <I/σ(I)> | 20 | 2.26 |
| Completeness [%] | 98.4 | 100 |
| Redundancy | 8.9 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | The crystals were grown using the in meso crystallization method |
