5BQY

Crystal structure of hemagglutinin of A/Chicken/Guangdong/S1311/2010 (H6N6) in complex with avian-like receptor LSTa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	77
Detector technology	CCD
Collection date	2014-10-24
Detector	RAYONIX MX-300
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	69.350, 137.770, 199.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.873 - 2.780
R-factor	0.1986
Rwork	0.196
R-free	0.25220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bny
RMSD bond length	0.004
RMSD bond angle	0.937
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	59.130
High resolution limit [Å]	2.780
Number of reflections	46081
<I/σ(I)>	11.3
Completeness [%]	99.7
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	0.1 M Bis-Tris propane (pH 8.0) and 2.3 M (NH4)2SO4