5BQX

Crystal structure of human STING in complex with 3'2'-cGAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Detector technology	CCD
Collection date	2014-12-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97940
Spacegroup name	P 41 21 2
Unit cell lengths	111.061, 111.061, 35.889
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.668 - 2.000
R-factor	0.1908
Rwork	0.187
R-free	0.22630
RMSD bond length	0.008
RMSD bond angle	1.039
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	15799
<I/σ(I)>	27.6
Completeness [%]	100.0
Redundancy	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	0.2 M ammonium acetate, 0.1 M trisodium citrate, pH 5.8, 25% PEG4000