5BQL

Fluorescent protein cyOFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.016230
Spacegroup name	P 21 21 21
Unit cell lengths	74.124, 102.764, 123.254
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.390
R-factor	0.1955
Rwork	0.193
R-free	0.25220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.826
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.000		2.530
High resolution limit [Å]	2.390	7.100	2.390
Rmerge	0.084	0.036	1.022
Rmeas	0.092	0.039	1.114
Total number of observations	467689
Number of reflections	38045	2759	11250
<I/σ(I)>	14.8	39.69	1.94
Completeness [%]	99.3	99.4	96.8
Redundancy	6.53

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M sodium chloride, 0.1M HEPES pH 7.5, 25% PEG 3350