5BQ7
Crystal structure of chikungunya virus-human Fab 5F-10 fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.207, 66.625, 168.928 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.000 - 2.738 |
| R-factor | 0.2377 |
| Rwork | 0.232 |
| R-free | 0.28840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v0v 1hzh 4d9l |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.827 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.810 |
| High resolution limit [Å] | 2.738 | 2.750 |
| Rmerge | 0.196 | 0.692 |
| Number of reflections | 11899 | |
| <I/σ(I)> | 7.25 | 1.39 |
| Completeness [%] | 94.4 | 94 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% 2-propanol, 25% PEG-4000, 50mM HEPES buffer pH 7.5 |
