5BPW

Atomic-resolution structures of the APC/C subunits Apc4 and the Apc5 N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.987
Spacegroup name	P 4 21 2
Unit cell lengths	139.181, 139.181, 156.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.943 - 3.400
R-factor	0.2208
Rwork	0.220
R-free	0.27490
RMSD bond length	0.012
RMSD bond angle	1.828
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.800	3.670
High resolution limit [Å]	3.400	3.400
Rmerge	0.182	0.600
Number of reflections	21671
<I/σ(I)>	5.16	2.3
Completeness [%]	99.8	99.6
Redundancy	6.5	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	Apc4 crystals were grown by vapour-diffusion in a buffer containing 10 mM malic acid, 20 mM MES, 20 mM Tris.HCl (pH 8.0), 160 mM NDSB 196, 10 mM EDTA and 19% (v/v) PEG 1500. Crystals were incubated in a cryoprotection buffer comprising 10 mM malic acid, 20 mM MES, 20 mM Tris.HCl (pH 8.0), 160 mM NDSB 196, 10 mM EDTA, 22% (v/v) PEG 1500 and 20% (v/v) ethylene glycol prior to freezing in liquid nitrogen.