5BPG
Crystal structure of the water-soluble FraC purified starting from the trans-membrane pore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.300, 44.440, 114.950 |
| Unit cell angles | 90.00, 92.82, 90.00 |
Refinement procedure
| Resolution | 47.190 - 2.140 |
| R-factor | 0.21269 |
| Rwork | 0.210 |
| R-free | 0.26286 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vwi |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.475 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Aimless |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.190 | 2.210 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.099 | 0.310 |
| Number of reflections | 42232 | |
| <I/σ(I)> | 9.7 | 3.4 |
| Completeness [%] | 97.5 | 89.3 |
| Redundancy | 4.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Microseeds generated in: 24% PEG 4000, 200 mM ammonium sulfate, 100 mM Cacodylate (pH 7.4) Single crystals generated in: 8% PEG 4000, 100 mM TRIS-HCl |
