5BOP
Crystal structure of the artificial nanobody octarellinV.1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.920, 62.860, 95.110 |
| Unit cell angles | 90.00, 96.22, 90.00 |
Refinement procedure
| Resolution | 8.720 - 1.950 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.22920 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.012 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.720 |
| High resolution limit [Å] | 1.950 |
| Number of reflections | 89920 |
| <I/σ(I)> | 8.51 |
| Completeness [%] | 97.5 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 100mM Tris pH 8.5, 22% PEG 3350, 10% EtOH, streak seeding |
