5BO4
Structure of SOCS2:Elongin C:Elongin B from DMSO-treated crystals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0432, 0.9537 |
| Spacegroup name | P 32 |
| Unit cell lengths | 185.217, 185.217, 67.204 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.300 - 2.900 |
| R-factor | 0.2317 |
| Rwork | 0.229 |
| R-free | 0.28720 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.014 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.300 | 46.300 | 2.980 |
| High resolution limit [Å] | 2.900 | 12.640 | 2.900 |
| Rmerge | 0.067 | 0.022 | 0.622 |
| Rmeas | 0.124 | ||
| Rpim | 0.039 | 0.013 | 0.360 |
| Total number of observations | 219197 | 2337 | 17340 |
| Number of reflections | 56676 | ||
| <I/σ(I)> | 15.6 | 107.7 | 120.9 |
| Completeness [%] | 99.2 | 92.6 | 100 |
| Redundancy | 3.9 | 3.7 | 3.9 |
| CC(1/2) | 0.999 | 0.999 | 0.673 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 291 | PEG 3350, CaOAc, Sodium cacodylate, Glycerol |
