5BO4

Structure of SOCS2:Elongin C:Elongin B from DMSO-treated crystals

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-31
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0432, 0.9537
Spacegroup name	P 32
Unit cell lengths	185.217, 185.217, 67.204
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.300 - 2.900
R-factor	0.2317
Rwork	0.229
R-free	0.28720
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.014
Data reduction software	XDS
Data scaling software	Aimless (0.3.11)
Phasing software	SOLVE
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.300	46.300	2.980
High resolution limit [Å]	2.900	12.640	2.900
Rmerge	0.067	0.022	0.622
Rmeas	0.124
Rpim	0.039	0.013	0.360
Total number of observations	219197	2337	17340
Number of reflections	56676
<I/σ(I)>	15.6	107.7	120.9
Completeness [%]	99.2	92.6	100
Redundancy	3.9	3.7	3.9
CC(1/2)	0.999	0.999	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	291	PEG 3350, CaOAc, Sodium cacodylate, Glycerol