5BNH
Crystal structure of the HLTF HIRAN domain with a ssDNA fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.469, 50.117, 102.763 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.380 - 1.700 |
| R-factor | 0.16719 |
| Rwork | 0.165 |
| R-free | 0.21353 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.933 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 51.380 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 28334 |
| <I/σ(I)> | 3.9 |
| Completeness [%] | 99.9 |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | 0.1 M Sodium acetate trihydrate pH 5.0, 40% 2-Methyl-2,4-pentanediol |
