5BNG
monomer of TALE type homeobox transcription factor MEIS1 complexes with specific DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.006370 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.900, 59.940, 107.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.919 - 3.500 |
| R-factor | 0.3451 |
| Rwork | 0.343 |
| R-free | 0.38810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.727 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 3.350 |
| High resolution limit [Å] | 3.230 | 3.230 |
| Rmerge | 0.340 | |
| Number of reflections | 9233 | |
| <I/σ(I)> | 6.9 | 1.3 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 1.1 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.09 | 293 | PEG 4000, magnesium chloride, PEG 400, HEPES |
