5BND

Crystal structure of the C-terminal domain of TagH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	104.576, 124.749, 56.132
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.413 - 2.680
R-factor	0.2085
Rwork	0.206
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.320
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.413
High resolution limit [Å]	2.680
Number of reflections	18143
<I/σ(I)>	3
Completeness [%]	99.2
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	12 % PEG 3350, 0.1 M malic acid (pH 7.0).