5B8C
High resolution structure of the human PD-1 in complex with pembrolizumab Fv
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 143.680, 143.130, 76.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.418 | 2.200 |
High resolution limit [Å] | 2.146 | 2.150 |
Rmerge | 0.094 | |
Number of reflections | 86668 | |
<I/σ(I)> | 15.5 | |
Completeness [%] | 99.6 | |
Redundancy | 6.5 | |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20 % PEG 3350, 0.2 M KNO3 |