5B8A
Crystal structure of oxidized chimeric EcAhpC1-186-YFSKHN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.999999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.470, 134.730, 107.530 |
| Unit cell angles | 90.00, 111.06, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.700 |
| R-factor | 0.24634 |
| Rwork | 0.244 |
| R-free | 0.28963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o5r |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.994 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 69616 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 96.1 | 91.3 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.6 M ammonium sulfate, 100 mM MES pH 6.5, 5% Dioxane |
