5B7G
Structures and functional analysis of periplasmic 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Aeromonas hydrophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 102.671, 102.671, 118.837 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.639 - 1.399 |
| R-factor | 0.1494 |
| Rwork | 0.149 |
| R-free | 0.16360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nm4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.088 |
| Data reduction software | HKL-2000 |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.490 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 142443 |
| <I/σ(I)> | 14.79 |
| Completeness [%] | 99.2 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.6 | 288 | 6% (w/v) polyethylene glycol 4000, 0.1 M sodium acetate trihydrate (pH 4.6) |
