5B79

Crystal structure of DPF2 double PHD finger

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-05
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	I 4 2 2
Unit cell lengths	106.979, 106.979, 52.592
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.599 - 2.601
R-factor	0.1941
Rwork	0.189
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4llb
RMSD bond length	0.006
RMSD bond angle	0.940
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.600	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	4902
<I/σ(I)>	29.9
Completeness [%]	99.6
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	ammonium citrate tribasic, Bis-Tris propane