5B77
Crystal structrue of MOZ double PHD finger in complex with histone H3 propionylation at K14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.708, 48.028, 75.168 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.800 - 1.551 |
R-factor | 0.1755 |
Rwork | 0.174 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4llb |
RMSD bond length | 0.011 |
RMSD bond angle | 1.257 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.800 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 25683 | |
<I/σ(I)> | 32.2 | |
Completeness [%] | 99.9 | |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | polyethylene glycol 4000, lithium sulfate, Tris |