5B76
Crystal structure of MOZ double PHD finger domain in complex with histone H3 crotonylation at K14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97893 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.120, 47.633, 76.109 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.060 - 1.653 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.23650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4llb |
RMSD bond length | 0.003 |
RMSD bond angle | 0.628 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.100 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 21315 | |
<I/σ(I)> | 44.2 | |
Completeness [%] | 99.5 | |
Redundancy | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | polyethylene glycol 4000, lithium sulfate, Tris |