5B73

Crystal structure of human ZMYND8 PHD-Bromo-PWWP domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9790
Spacegroup name	P 21 21 21
Unit cell lengths	67.083, 67.999, 70.309
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.500 - 1.800
R-factor	0.1886
Rwork	0.187
R-free	0.22430
RMSD bond length	0.012
RMSD bond angle	1.258
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.500
High resolution limit [Å]	1.800
Number of reflections	30158
<I/σ(I)>	22.4
Completeness [%]	99.3
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	HEPES, sodium citrate tribasic