5B70
OxyR2 E204G regulatory domain from Vibrio vulnificus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-13 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97933 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 141.375, 141.375, 109.664 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.957 - 2.300 |
R-factor | 0.2293 |
Rwork | 0.228 |
R-free | 0.26750 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.586 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.960 | 2.620 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.089 | 0.368 |
Number of reflections | 53831 | |
<I/σ(I)> | 9.67 | 2.46 |
Completeness [%] | 95.3 | 88 |
Redundancy | 5.6 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 287 | 0.2M sodium acetate, 0.1M Tris-HCl (pH 9.0), 14% PEG 4000, 2mM TCEP |