5B5Z

Crystal structure of PtLCIB4 H88A mutant, a homolog of the limiting CO2-inducible protein LCIB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	48.079, 48.124, 59.922
Unit cell angles	76.77, 87.96, 86.05

Refinement procedure

Resolution	47.955 - 1.600
R-factor	0.1806
Rwork	0.179
R-free	0.20720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b5y
RMSD bond length	0.007
RMSD bond angle	1.109
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.960	1.690
High resolution limit [Å]	1.600	1.600
Number of reflections	68811
<I/σ(I)>	18.6
Completeness [%]	95.1
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	200 mM sodium acetate trihydrate, 100 mM Tris, 27% w/v PEG4000