5B5R
Crystal structure of GSDMA3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2015-09-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.453, 103.414, 49.625 |
| Unit cell angles | 90.00, 110.57, 90.00 |
Refinement procedure
| Resolution | 37.914 - 1.902 |
| R-factor | 0.1893 |
| Rwork | 0.187 |
| R-free | 0.23200 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.848 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.071 | 0.876 |
| Number of reflections | 44110 | |
| <I/σ(I)> | 25.6 | 2.4 |
| Completeness [%] | 97.1 | 92.7 |
| Redundancy | 6.6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM Bis-Tris (pH 6.5), 19% polyethylene glycol 3550, 10 mM TCEP |
