5B4S
Crystal Structure of GH80 chitosanase from Mitsuaria chitosanitabida
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-08 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.02 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.879, 56.366, 207.081 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.659 - 1.750 |
| R-factor | 0.1612 |
| Rwork | 0.160 |
| R-free | 0.19170 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.037 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.660 | 1.812 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.057 | 0.139 |
| Number of reflections | 61905 | |
| <I/σ(I)> | 23.74 | 12.38 |
| Completeness [%] | 99.3 | 98.65 |
| Redundancy | 7.2 | 7.2 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | PEG 4000, ammonium sulphate, sodium acetate |
