5B4J
Crystal structure of I86D mutant of phycocyanobilin:ferredoxin oxidoreductase in complex with biliverdin (data 3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-25 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.800 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.823, 95.861, 42.378 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.050 |
| Rwork | 0.139 |
| R-free | 0.16770 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4qcd |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.032 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.970 | 1.090 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.378 | |
| Number of reflections | 134801 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | sodium citrate, sodium chloride, Tris |
