5B4F

Sulfur Transferase TtuA in complex with iron sulfur cluster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-24
Detector	RAYONIX MX225HE
Wavelength(s)	0.98
Spacegroup name	P 61 2 2
Unit cell lengths	93.920, 93.920, 264.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.960 - 2.750
R-factor	0.2212
Rwork	0.219
R-free	0.25720
RMSD bond length	0.010
RMSD bond angle	1.319
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.750
Number of reflections	18794
<I/σ(I)>	21.6
Completeness [%]	99.9
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M sodium acetate (pH 3.8-5.6), 30-60%(v/v) 1,2-propanediol