5B49
Crystal structure of LpxH with manganese from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-17 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000, 1.8925 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.937, 108.862, 51.555 |
| Unit cell angles | 90.00, 92.68, 90.00 |
Refinement procedure
| Resolution | 29.230 - 1.650 |
| R-factor | 0.15288 |
| Rwork | 0.152 |
| R-free | 0.17662 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.403 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 64565 | |
| <I/σ(I)> | 20.4 | 2.23 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 0.2M L-Proline, 0.1M HEPES pH 7.6, 8.5% PEG 3350 |
