5B42

Crystal structure of the C-terminal endonuclease domain of Aquifex aeolicus MutL.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-02
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	35.349, 35.349, 161.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.702 - 1.350
R-factor	0.1422
Rwork	0.140
R-free	0.18340
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.168
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.702	1.400
High resolution limit [Å]	1.350	1.350
Rmerge	0.056
Number of reflections	23801
<I/σ(I)>	59.8
Completeness [%]	100.0
Redundancy	13.5
CC(1/2)	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293.15	14.7mg/ml protein, 100mM sodium acetate, 50mM cadmium chloride, and 15%(v/v) polyethylene glycol 400