5B31

The crystal structure of the heterotypic H2AZ/H2A nucleosome with H3.1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-25
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	105.110, 109.685, 181.487
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.622 - 2.200
R-factor	0.2277
Rwork	0.225
R-free	0.27070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3afa
RMSD bond length	0.010
RMSD bond angle	1.121
Data scaling software	HKL-2000 (705b)
Phasing software	PHASER (2.5.1)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.093	0.062	0.499
Number of reflections	105458
<I/σ(I)>	12.7
Completeness [%]	97.9	99.7	93.7
Redundancy	4.6	6.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	potassium cacodylate, potassium chloride, manganese chloride